Zinc sparks are triggered by fertilization and facilitate cell cycle resumption in mammalian eggs.
ACS Chem Biol. 2011 Jul 15;6(7):716-23
Authors: Kim AM, Bernhardt ML, Kong BY, Ahn RW, Vogt S, Woodruff TK, O'Halloran TV
Abstract In last few hours of maturation, the mouse oocyte takes up over twenty billion…
In vitro growth and steroidogenesis of dog follicles are influenced by the physical and hormonal microenvironment.
Reproduction. 2011 Jul;142(1):113-22
Authors: Songsasen N, Woodruff TK, Wildt DE
Abstract The present study examined the influences of the physical and hormonal microenvironment on in vitro growth and steroidogenesis of dog follicles. Follicles were…
A macrophage and theca cell-enriched stromal cell population influences growth and survival of immature murine follicles in vitro.
Reproduction. 2011 Jun;141(6):809-20
Authors: Tingen CM, Kiesewetter SE, Jozefik J, Thomas C, Tagler D, Shea L, Woodruff TK
Abstract Innovations in in vitro ovarian follicle culture have revolutionized the field of fertility…
The time is now for a new approach to primary ovarian insufficiency.
Fertil Steril. 2011 May;95(6):1890-7
Authors: Cooper AR, Baker VL, Sterling EW, Ryan ME, Woodruff TK, Nelson LM
Abstract OBJECTIVE: To articulate the need for a new approach to primary ovarian insufficiency. The condition, also known as premature menopause…
In vitro oocyte maturation and preantral follicle culture from the luteal-phase baboon ovary produce mature oocytes.
Biol Reprod. 2011 Apr;84(4):689-97
Authors: Xu M, Fazleabas AT, Shikanov A, Jackson E, Barrett SL, Hirshfeld-Cytron J, Kiesewetter SE, Shea LD, Woodruff TK
Abstract Female cancer patients who seek fertility preservation but cannot undergo…
Hydrogel network design using multifunctional macromers to coordinate tissue maturation in ovarian follicle culture.
Biomaterials. 2011 Apr;32(10):2524-31
Authors: Shikanov A, Smith RM, Xu M, Woodruff TK, Shea LD
Abstract Synthetic hydrogels with tunable properties are appealing for regenerative medicine. A critical limitation in hydrogel design at low solids concentration is…
Structure of betaglycan zona pellucida (ZP)-C domain provides insights into ZP-mediated protein polymerization and TGF-beta binding.
Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5232-6
Authors: Lin SJ, Hu Y, Zhu J, Woodruff TK, Jardetzky TS
Abstract The zona pellucida (ZP) domain is a bipartite protein structural element comprised…
Zinc requirement during meiosis I-meiosis II transition in mouse oocytes is independent of the MOS-MAPK pathway.
Biol Reprod. 2011 Mar;84(3):526-36
Authors: Bernhardt ML, Kim AM, O'Halloran TV, Woodruff TK
Abstract Zinc is essential for many biological processes, including proper functioning of gametes. We recently reported that zinc levels rise by…
Results from the survey for preservation of adolescent reproduction (SPARE) study: gender disparity in delivery of fertility preservation message to adolescents with cancer.
J Assist Reprod Genet. 2011 Mar;28(3):269-77
Authors: Köhler TS, Kondapalli LA, Shah A, Chan S, Woodruff TK, Brannigan RE
Abstract PURPOSE: Diminished reproductive capacity is a devastating…
Role of PCSK5 expression in mouse ovarian follicle development: identification of the inhibin α- and β-subunits as candidate substrates.
PLoS One. 2011;6(3):e17348
Authors: Antenos M, Lei L, Xu M, Malipatil A, Kiesewetter S, Woodruff TK
Abstract Inhibin and activin are essential dimeric glycoproteins belonging to the transforming growth factor-beta (TGFβ)…
A new hypothesis regarding ovarian follicle development: ovarian rigidity as a regulator of selection and health.
J Assist Reprod Genet. 2011 Jan;28(1):3-6
Authors: Woodruff TK, Shea LD
Abstract The mammalian ovary consists of a large number of dormant immature follicles, each containing a single oocyte and located on the periphery…
A method for ovarian follicle encapsulation and culture in a proteolytically degradable 3 dimensional system.
J Vis Exp. 2011;(49)
Authors: Shikanov A, Xu M, Woodruff TK, Shea LD
Abstract The ovarian follicle is the functional unit of the ovary that secretes sex hormones and supports oocyte maturation. In vitro follicle…
The Gynecologist Has a Unique Role in Providing Oncofertility Care to Young Cancer Patients.
US Obstet Gynecol. 2011 Jan 1;6(1):24-34
Authors: Duncan FE, Jozefik JK, Kim AM, Hirshfeld-Cytron J, Woodruff TK
Abstract Facing a cancer diagnosis at any age is devastating. However, young cancer patients have the added burden that…
Incorporating fertility preservation into the care of young oncology patients.
Cancer. 2011 Jan 1;117(1):4-10
Authors: Redig AJ, Brannigan R, Stryker SJ, Woodruff TK, Jeruss JS
Abstract As the number of cancer survivors continues to increase, oncologists are faced with the challenge of providing cancer therapy to patients who may 1…
Research funding. Politics and parthenotes.
Science. 2010 Oct 22;330(6003):453
Authors: Tingen C, Rodriguez S, Campo-Engelstein L, Woodruff TK
PMID: 20966235 [PubMed - indexed for MEDLINE]
Zinc availability regulates exit from meiosis in maturing mammalian oocytes.
Nat Chem Biol. 2010 Sep;6(9):674-81
Authors: Kim AM, Vogt S, O'Halloran TV, Woodruff TK
Abstract Cellular metal ion fluxes are known in alkali and alkaline earth metals but are not well documented in transition metals. Here we describe major changes…
The Oncofertility Consortium--addressing fertility in young people with cancer.
Nat Rev Clin Oncol. 2010 Aug;7(8):466-75
Authors: Woodruff TK
Abstract The number of young cancer survivors is increasing owing to advances in cancer therapeutics, but many face infertility as a result of their treatment. Technologies that already exist for cancer patients…
Current achievements and future research directions in ovarian tissue culture, in vitro follicle development and transplantation: implications for fertility preservation.
Hum Reprod Update. 2010 Jul-Aug;16(4):395-414
Authors: Smitz J, Dolmans MM, Donnez J, Fortune JE, Hovatta O, Jewgenow K, Picton HM, Plancha C, Shea LD, Stouffer RL, Telfer EE, Woodruff TK, Zelinski MB
Abstract
BACKGROUND Female cancer patients are offered…
Sex and sensitivity: the continued need for sex-based biomedical research and implementation.
Womens Health (Lond Engl). 2010 Jul;6(4):511-6
Authors: Tingen CM, Kim AM, Wu PH, Woodruff TK
Abstract The phrase 'women's health research' embraces women as part of the biomedical research engine while categorizing women as separate. Before personalized medicine…
Setting the course: leadership at the NICHD.
Biol Reprod. 2010 Jul;83(1):163-4
Authors: Woodruff TK
PMID: 20505167 [PubMed - indexed for MEDLINE]
Sex bias in trials and treatment must end.
Nature. 2010 Jun 10;465(7299):688-9
Authors: Kim AM, Tingen CM, Woodruff TK
PMID: 20535184 [PubMed - indexed for MEDLINE]
Noninvasive index of cryorecovery and growth potential for human follicles in vitro.
Biol Reprod. 2010 Jun;82(6):1180-9
Authors: Barrett SL, Shea LD, Woodruff TK
Abstract Cryopreservation of oocytes and embryos is commonly used to preserve fertility. However, women undergoing cancer treatment may not have the time or may not be good…
A novel two-step strategy for in vitro culture of early-stage ovarian follicles in the mouse.
Fertil Steril. 2010 May 15;93(8):2633-9
Authors: Jin SY, Lei L, Shikanov A, Shea LD, Woodruff TK
Abstract OBJECTIVE: To develop an in vitro strategy to support the growth of early-stage follicles and produce mature oocytes…
Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation.
Fertil Steril. 2010 May 15;93(8):2627-32
Authors: Jin S, Lei L, Shea LD, Zelinski MB, Stouffer RL, Woodruff TK
Abstract OBJECTIVE: To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles…